Murine memory B cells are multi-isotype expressors.

C J Wu; J T Karttunen; D H Chin; D Sen; W Gilbert

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PubMed · 1997400

Murine memory B cells are multi-isotype expressors.

C J Wu·J T Karttunen·D H Chin·D Sen·W Gilbert

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Abstract

Flow cytometric analyses of the surface immunoglobulins of murine memory B cells revealed the existence of populations expressing multiple isotypes, including an IgM+/IgG+ population that could be stimulated in vitro with antigen to secrete both IgM and IgG. Female BALB/c mice were immunized with R-phycoerythrin (RPE), a fluorescent photosynthetic accessory protein from red algae. Pooled splenocytes from these mice at different stages of immunization were stained with RPE as well as with allophycocyanin- and fluorescein-conjugated anti-isotype antibodies and analysed on a two-laser FACS. RPE-binding cell sub-populations were defined and selectively sorted to verify their phenotype and to demonstrate that the various subpopulations (IgM+/IgG+, IgM+/IgG-, IgM-/IgG+) had different isotype-secretion patterns when challenged with RPE in vitro. These results re-affirm the notion that a transcriptional processing mechanism may be responsible for the simultaneous expression of multiple isotypes in memory cells.

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Animals B-Lymphocyte Subsets Female Immunoglobulin D Immunoglobulin G Immunoglobulin Isotypes Immunoglobulin M Immunologic Memory Mice Mice, Inbred BALB C Phycoerythrin Journal Article

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C J Wu, J T Karttunen, D H Chin, D Sen, W Gilbert. 1991. Murine memory B cells are multi-isotype expressors.. https://pubmed.ncbi.nlm.nih.gov/1997400/

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