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Cellular immunity in current active pulmonary tuberculosis.

R Andrade-Arzabe·I V Machado·B Fernandez·I Blanca·R Ramirez·N E Bianco
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Abstract

A group of 10 patients with recently diagnosed pulmonary TB were studied and compared to 10 bacillus Calmette-Guérin (BCG) immunized healthy individuals. Cellular immune mechanisms were explored in vitro utilizing fresh and precultured peripheral blood mononuclear cells exposed to PHA, PPD, and recall antigens (SK/SD and CA). Proliferative assays were also carried out in the presence of either each patient's serum (autologous serum) or cocultured with CD3(+)-depleted adherent cells. Serum measurements of soluble interleukin-2 (IL-2) receptor and synthesis of IL-2 generated by mononuclear cells stimulated with PPD and SK/SD were also performed. Patient sera were able to inhibit autologous as well as allogeneic cell responses, and a significant adherent cell suppressive effect was observed. As a whole the group of patients showed decreased blast transformation to PPD, preserved proliferative responses to other recall antigens, and a low PPD-induced generation of IL-2. Furthermore, as possible evidence of preactivated T cells, these patients demonstrated high soluble IL-2 receptor serum levels. Early compromise of specific cell-mediated immunity, including IL-2 abnormalities, may be of significance in newly diagnosed pulmonary TB.

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AdultFemaleHumansImmunity, CellularInterleukin-2Leukocytes, MononuclearLymphocyte ActivationMaleMiddle AgedReceptors, Interleukin-2T-Lymphocyte SubsetsTuberculosis, PulmonaryJournal ArticleResearch Support, Non-U.S. Gov't

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R Andrade-Arzabe, I V Machado, B Fernandez, I Blanca, R Ramirez, N E Bianco. 1991. Cellular immunity in current active pulmonary tuberculosis.. https://doi.org/10.1164/ajrccm%2F143.3.496

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