Rapid polymerase chain reaction amplification using intact bacterial cells.

A K Joshi; V Baichwal; G F Ames

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PubMed · 2003920

Rapid polymerase chain reaction amplification using intact bacterial cells.

A K Joshi·V Baichwal·G F Ames

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Abstract

We have demonstrated that efficient polymerase chain reaction amplifications from chromosomal DNA can be carried out using whole bacterial cells as the starting material. Cells from the liquid or solid cultures can be used directly, without any pre-treatment, thus eliminating the need for DNA isolation.

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ATP-Binding Cassette Transporters Amino Acid Transport Systems, Basic Bacterial Proteins Chromosomes, Bacterial DNA, Bacterial Escherichia coli Membrane Transport Proteins Operon Polymerase Chain Reaction Salmonella typhimurium Research Support, U.S. Gov't, P.H.S.Technical Report Journal Article

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A K Joshi, V Baichwal, G F Ames. 1991. Rapid polymerase chain reaction amplification using intact bacterial cells.. https://pubmed.ncbi.nlm.nih.gov/2003920/

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