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PubMed · 2005357

Microassay for DNA methyltransferase.

R L Adams·A Rinaldi·C Seivwright
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Abstract

A microassay for DNA methylase is described which can detect activity in as few as 50 tissue culture cells. The cells are lysed and incubated for 2 h at 37 degrees C with 3 microCi high specific activity [3H]AdoMet and 0.5 microgram poly[d(I-C).d(I-C)] in a volume of 23 microliters. Ribonuclease is present during the assay and the product DNA is isolated by phenol extraction after protease digestion.

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DNA Modification MethylasesKineticsMicrochemistrySubstrate SpecificityJournal ArticleResearch Support, Non-U.S. Gov't

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R L Adams, A Rinaldi, C Seivwright. 1991. Microassay for DNA methyltransferase.. https://doi.org/10.1016/0165-022x(91)90077-a

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