Rat small intestinal mucins: a quantitative analysis.

Abstract

Although much is known about the qualitative distribution of mucin-secreting goblet cells in the small intestine, the quantitative distribution of stored mucins remains undefined. The purpose of this study was to determine the distribution of neutral stored mucin in the rat small intestine by using morphometric techniques and once established, to verify that this methodology could detect secretion in animals exposed to a known mucin secretagogue. Twelve male Wistar rats (five baseline, five pilocarpine-treated, and two vehicle controls) were fixed by vascular perfusion. After a brief fixation the intestine was removed, cut into 10 equal segments, sliced, and fixed overnight. Methacrylate sections from each segment were stained with periodic acid-Schiff and toluidine blue. For morphometry, the volume of epithelium per surface area of epithelial basal lamina was calculated with a Merz grid. The volume density of stored mucin per epithelium was determined by point-counting on a square lattice grid. Volumes were related to either surface area of epithelial basal lamina or mucosal surface area. Due mostly to contributions by villus stored mucin, the total amount of product was found to increase proximally to distally in the small bowel, with the most dramatic increases occurring in the first three segments. When subjected to pilocarpine, a massive secretory response was evoked, resulting in a near total depletion of crypt stored mucin at all levels of the small bowel. Secretion of villus stored mucin also occurred throughout the small intestine, however reaching levels of significance at only a few points. This study describes the distribution of stored mucin in the small intestine under baseline and accelerated secretory conditions.