Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.

J Welsh; C Petersen; M McClelland

doi:10.1093/nar/19.2.303

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Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.

J Welsh·C Petersen·M McClelland

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Abstract

Polymorphisms in genomic fingerprints generated by arbitrarily primed PCR (AP-PCR) can distinguish between strains of almost any organism. We applied the technique to the mouse (Mus musculus). The characteristic differences in the AP-PCR genomic fingerprints between strains will be of value in strain identification and verification. Using one primer, we genetically mapped four polymorphisms in a set of C57BL/6J x DBA/2J recombinant inbreds. One of these polymorphisms is a length variant. The method will allow rapid genetic mapping of DNA polymorphisms without Southern blotting.

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Animals Autoradiography Base Sequence DNA DNA Fingerprinting Electrophoresis, Polyacrylamide Gel Mice Mice, Inbred Strains Molecular Sequence Data Polymerase Chain Reaction Polymorphism, Genetic Recombination, Genetic Species Specificity Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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J Welsh, C Petersen, M McClelland. 1991-01-25. Polymorphisms generated by arbitrarily primed PCR in the mouse: application to strain identification and genetic mapping.. https://doi.org/10.1093/nar%2F19.2.303

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