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PubMed · 2029527

Structurally different rat liver medium-chain acyl CoA dehydrogenases directed by complementary DNAs differing in their 5'-region.

T Inagaki·N Ohishi·N Tsukagoshi·S Udaka·S Ghisla·K Yagi
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Abstract

Different forms of rat liver medium-chain acyl CoA dehydrogenase (MCAD) (EC 1.3.99.3) were produced in Escherichia coli carrying expression plasmids (pRMCADm-1 approximately 9) differing at the 5'-region of the cDNA. The proteins expressed could be readily extracted from the cells. The protein (approximately 44 kDa) directed by pRMCADm-3 showed the highest activity and was readily purified to homogeneity. The purified enzyme contained non-covalently bound FAD and was similar to rat liver mitochondrial enzyme in all respects examined. The purified protein (approximately 45 kDa) directed by pRMCADm-1 did not contain FAD and showed no enzymatic activity. Therefore, the leader peptide disturbs the binding of FAD to the apoprotein. The purified protein (approximately 40 kDa) directed by pRMCADm-6 did not contain FAD. Thus, the deletion of the NH2-terminal portion of the apoprotein to some extent results in its inability to combine with FAD.

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Acyl-CoA DehydrogenaseAcyl-CoA DehydrogenasesAmino Acid SequenceAnimalsBase SequenceDNAEscherichia coliLiverMolecular Sequence DataRatsJournal Article

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T Inagaki, N Ohishi, N Tsukagoshi, S Udaka, S Ghisla, K Yagi. 1991-04-29. Structurally different rat liver medium-chain acyl CoA dehydrogenases directed by complementary DNAs differing in their 5'-region.. https://doi.org/10.1016/0167-4838(91)90542-8

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PubMed2006

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