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PubMed · 2037105

Platelet-activating factor induces progesterone secretion and changes in morphological appearance in luteinizing granulosa cells in vitro.

J Rabinovici·M J Angle
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Abstract

OBJECTIVE: Recent evidence indicates the involvement of platelet-activating factor (PAF) in ovarian periovulatory processes. The objective of this study was to investigate the influence of PAF on ovarian follicular cellular function. DESIGN AND INTERVENTIONS: We examined the effects of PAF on progesterone (P) secretion by cultured human luteinizing granulosa cells obtained from women undergoing in vitro fertilization. Follicular cells were cultured in serum-free conditions for 12 to 36 hours with and without PAF (at dosages of 10(-8) to 10(-14) M). RESULTS: Progesterone secretion was significantly increased at 12 and 36 hours after treatment with 10(-8) M PAF (170% +/- 40% and 210% +/- 40%, respectively) and with 10(-10) M PAF (170% +/- 30% and 180% +/- 10%, respectively). Smaller increases were seen with 10(-12) and 10(-14) M PAF. Maximal increases in P secretion in 10(-10) M PAF-treated wells occurred at 3 to 12 hours. At 36 hours, distinct morphological differences between cells in control wells and those containing PAF were noted. CONCLUSIONS: These results indicate that PAF may influence follicular cell function during ovulation and corpus luteum formation.

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Cells, CulturedFemaleFertilization in VitroGranulosa CellsHumansKineticsPlatelet Activating FactorProgesteroneJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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J Rabinovici, M J Angle. 1991. Platelet-activating factor induces progesterone secretion and changes in morphological appearance in luteinizing granulosa cells in vitro.. https://doi.org/10.1016/s0015-0282(16)54360-x

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