Two hypervariable minisatellite DNA binding proteins.

W P Wahls; G Swenson; P D Moore

doi:10.1093/nar/19.12.3269

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Two hypervariable minisatellite DNA binding proteins.

W P Wahls·G Swenson·P D Moore

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Abstract

Hypervariable minisatellite DNA sequences are short, tandemly repeated sequences present at numerous loci in eukaryotes. They stimulate intermolecular homologous recombination up to 13-fold in human cells in culture and may be specific sites for the initiation of recombination in the eukaryotic genome (Wahls, W.P., Wallace, L.J., & Moore, P.D. (1990) Cell 60, 95-103). Reported here is the detection and partial purification of two hypervariable minisatellite DNA binding proteins, called Msbp-2 and Msbp-3, present in the nuclear extracts of human HeLa cells. The proteins elute from a gel filtration column with a native mass of 200-250 kDa and have sizes of 77 kDa and 115 kDa respectively.

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Base Sequence Chromatography, Affinity Chromatography, Ion Exchange DNA DNA, Satellite DNA-Binding Proteins Electrophoresis, Polyacrylamide Gel HeLa Cells Humans Molecular Sequence Data Nucleic Acid Hybridization Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

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W P Wahls, G Swenson, P D Moore. 1991-06-25. Two hypervariable minisatellite DNA binding proteins.. https://doi.org/10.1093/nar%2F19.12.3269

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