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PubMed · 2062652

A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.

J Van Ness·S Kalbfleisch·C R Petrie·M W Reed·J C Tabone·N M Vermeulen
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Abstract

A procedure for immobilization of well-defined quantities of oligodeoxyribonucleotides (ODNs) to a versatile nylon support is described. The solid support, a nylon-6/6 bead, is covalently coated with poly(ethyleneimine) to provide a reactive spacer-arm for attachment of ODNs. 5'-Aminohexyl-tailed ODNs are selectively activated using 2,4,6-trichloro-1,3,5-triazine (cyanuric chloride) and then covalently attached to the bead via the triazine moiety. The modified nylon support has a low level of binding of nonspecific nucleic acid and efficiently captures both RNA and DNA targets.

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AlkylationBase SequenceColorimetryDNAFluorescenceGenetic TechniquesMolecular Sequence DataNucleic Acid HybridizationNylonsOligonucleotide ProbesPolyethyleneimineTriazinesJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J Van Ness, S Kalbfleisch, C R Petrie, M W Reed, J C Tabone, N M Vermeulen. 1991-06-25. A versatile solid support system for oligodeoxynucleotide probe-based hybridization assays.. https://doi.org/10.1093/nar%2F19.12.3345

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