Epidermal growth factor induced membrane changes in 3T3 cells.

A Aharonov; I Vlodavsky; R M Pruss; C F Fox; H R Herschman

doi:10.1002/jcp.1040950209

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Epidermal growth factor induced membrane changes in 3T3 cells.

A Aharonov·I Vlodavsky·R M Pruss·C F Fox·H R Herschman

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Abstract

Epidermal growth factor (EGF) is a mitogen for Swiss 3T3 cells. Short incubation periods with physiological concentrations of EGF induced increased binding of Swiss 3T3 cells to Con A-coated nylon fibers. This effect was not induced in an EGF non-responsive 33 variant, in the transformed murine XC cells or in Swiss SV3T3 cells. The increase in Con A fiber-binding seems to be specific for EGF, since it was not observed in response to insulin, prostaglandin F2alpha or a higher serum concentration, which also initiate cell devision of confluent quiescent 3T3 cells. EGF also reduced Con A-mediated hemadsorption to 3T3, but had no effect on hemadsorption by the EFG non-responsive 3T3 variant. There was no change in the number of Con A-receptors on 3T3 cells after EGF treatment. Binding to WGA-coated fibers and WGA-mediated hemadsorption were not effected by preincubation with EGF.

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Clone Cells Epidermal Growth Factor Lectins Mitogens Peptides Plant Lectins Receptors, Concanavalin A Receptors, Drug Simian virus 40 Time Factors Triticum Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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A Aharonov, I Vlodavsky, R M Pruss, C F Fox, H R Herschman. 1978. Epidermal growth factor induced membrane changes in 3T3 cells.. https://doi.org/10.1002/jcp.1040950209

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