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Yeast artificial chromosome vectors for efficient clone manipulation and mapping.

J H Shero·M K McCormick·S E Antonarakis·P Hieter
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Abstract

The yeast artificial chromosome (YAC) cloning system allows the cloning of exogenous DNA several hundred kilobases in length. To enhance the usefulness of this technology, yeast artificial chromosome vectors have been designed for efficient clone characterization, manipulation, and mapping. The vectors contain a polylinker with unique EcoRI, BglII, NotI, EagI, SacII, SalI, NruI, NheI, and ClaI cloning sites and T7 bacteriophage promoters positioned to allow the generation of riboprobes from the exogenous DNA ends. Centric and acentric vector arms were constructed as separate plasmids to allow the recovery of both ends of the YAC insert DNA directly in Escherichia coli. In addition, YACs generated using this vector system contain a yeast gene (SUP 11) that allows visual monitoring of YAC stability and copy number.

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AnimalsBase SequenceChromosome MappingChromosomes, FungalCloning, MolecularDNAEscherichia coliGene LibraryGenes, SyntheticGenetic VectorsMolecular Sequence DataRestriction MappingTetrahymenaJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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J H Shero, M K McCormick, S E Antonarakis, P Hieter. 1991. Yeast artificial chromosome vectors for efficient clone manipulation and mapping.. https://doi.org/10.1016/0888-7543(91)90343-d

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