Fragment tagging.

J H Zeilstra-Ryalls; R L Somerville

doi:10.1016/0735-0651(90)90029-f

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Fragment tagging.

J H Zeilstra-Ryalls·R L Somerville

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Abstract

A tactic known as fragment tagging, which has proven to be exceptionally useful in expediting DNA cloning and plasmid construction schemes, is described. The advantage of fragment tagging is that it facilitates the isolation of specific plasmid DNA molecules present in small amounts within mixed pools of DNA. Four examples that illustrate several variations of the fragment tagging concept are presented.

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Cloning, Molecular DNA DNA Ligases Drug Resistance Genetic Markers Plasmids Restriction Mapping Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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J H Zeilstra-Ryalls, R L Somerville. 1990. Fragment tagging.. https://doi.org/10.1016/0735-0651(90)90029-f

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