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PubMed · 2089063

Post-column continuous-flow analysis combined with reversed-phase liquid chromatography and computer-aided detection for the characterisation of peptides.

A F Fell·J B Castledine·B Sellberg·R Modin·R Weinberger
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Abstract

Peptide mapping is a key technique for structural identification of new proteins or the products of recombinant gene technology. The recognition of oligopeptides, separated by reversed-phase liquid chromatography, is limited by the conventional reliance on the correlation of retention times with standards, supported by dual-wavelength chromatograms. It has been reported that the recognition of phenolic compounds can be achieved by a novel technique, based on computer-aided photodiode-array detection of the pH-shifted solutes after post-column continuous-flow analysis. This work describes how the generation of the pH-shifted difference spectra for dipeptides, containing a tyrosyl residue, may be used to enhance peak recognition, when used in conjunction with absorbance ratios.

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Absorptiometry, PhotonAmino AcidsChromatography, LiquidElectronic Data ProcessingHydrogen-Ion ConcentrationPeptidesJournal ArticleResearch Support, Non-U.S. Gov't

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A F Fell, J B Castledine, B Sellberg, R Modin, R Weinberger. 1990-12-28. Post-column continuous-flow analysis combined with reversed-phase liquid chromatography and computer-aided detection for the characterisation of peptides.. https://doi.org/10.1016/s0021-9673(01)88933-0

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