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PubMed · 210982

A fluorogenic substrate for angiotensin-converting enzyme in plasma.

F S Russo·A V Persson·I B Wilson
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Abstract

A simple, sensitive, and reproducible assay for angiotensin-converting enzyme is described. It is based on the hydrolysis of the minimally fluorescent substrate p-nitrobenzyloxycarbonylglycyl-L-tryptophylglycine to the products p-nitrobenzyloxycarbonylglycine and the highly fluorescent L-tryptophylglycine. The L-tryptophylglycine was analyzed by fluorometry (lambda excitation = 285 nm; lambda emission = 350 nm). The mean value for human plasma (serum) is 16.5 nmol of substrate hydrolyzed per minute per milliter of plasma under the described assay conditions.

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DipeptidesFluorescent DyesHumansKineticsNitrobenzenesPeptidyl-Dipeptidase ASpectrometry, FluorescenceTryptophanJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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F S Russo, A V Persson, I B Wilson. 1978. A fluorogenic substrate for angiotensin-converting enzyme in plasma.. https://pubmed.ncbi.nlm.nih.gov/210982/

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