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A transferable plasmid associated with AS-48 production in Enterococcus faecalis.

M Martínez-Bueno·A Gálvez·E Valdivia·M Maqueda
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Abstract

Enterococcus faecalis S-48 produces a peptide antibiotic, AS-48, and a bacteriocin, Bc-48. We have isolated mutants that lack these inhibitory characteristics. Further analysis of the mutants indicates that a plasmid of 56 kilobases (pMB2) may harbor the genes for AS-48. In conjugation experiments, pMB2 has been transferred into a plasmid-free OG1X strain of E. faecalis. The OG1X(pMB2) transconjugant produces the antibiotic AS-48 in solid medium, and the MIC of AS-48 for this strain is the same as that of the donor strain.

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Anti-Bacterial AgentsBacterial ProteinsConjugation, GeneticDNA, BacterialEnterococcus faecalisEscherichia coliMutationPeptidesPhenotypePlasmidsJournal ArticleResearch Support, Non-U.S. Gov't

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M Martínez-Bueno, A Gálvez, E Valdivia, M Maqueda. 1990. A transferable plasmid associated with AS-48 production in Enterococcus faecalis.. https://doi.org/10.1128/jb.172.5.2817-2818.1990

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