A simple serum galactosyltransferase assay method suitable for routine use.

F Sichel; J P Malas; P Gauduchon; M Aubert; E Bar; J Y Le Talaër

doi:10.1016/0009-8981(90)90145-i

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A simple serum galactosyltransferase assay method suitable for routine use.

F Sichel·J P Malas·P Gauduchon·M Aubert·E Bar·J Y Le Talaër

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Abstract

UDP-Galactose: N-acetylglucosaminyl glycoprotein beta 1-4 galactosyltransferase (GT) catalyzes the transfer of galactose to N-acetylglucosamine from UDP-[3H]Gal. The uncharged reaction product (tritiated N-acetyllactosamine) is separated from the unreacted UDP-[3H]Gal by ion-exchange chromatography. The major advantage of this method is its rapidity compared to other isotopic techniques.

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Chromatography, Ion Exchange Female Humans Lactose Synthase Male N-Acetyllactosamine Synthase Reference Values Journal Article Research Support, Non-U.S. Gov't

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F Sichel, J P Malas, P Gauduchon, M Aubert, E Bar, J Y Le Talaër. 1990-04-13. A simple serum galactosyltransferase assay method suitable for routine use.. https://doi.org/10.1016/0009-8981(90)90145-i

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