Rabbit Ig kappa 1b6 gene structure.

K L Dreher; V Asundi; B Wolf; L Bruggeman

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PubMed · 2113556

Rabbit Ig kappa 1b6 gene structure.

K L Dreher·V Asundi·B Wolf·L Bruggeman

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Abstract

Previous studies employing Southern blot analyses have detected multiple kappa-homologous sequences within EcoRI-digested DNA isolated from kappa 1b6 homozygous rabbits and kappa 1b6 L chain secreting RMH H158 cell line. These results are very unexpected because the published partial protein sequence for the kappa 1b6 C region is incompatible with an EcoRI restriction endonuclease recognition sequence at the nucleotide level for this allotype. To determine their identity, the kappa-homologous sequences were isolated from DNA extracted from a kappa 1b6 L chain secreting RMH H158 cell line by molecular cloning. Structural analyses demonstrated these sequences to contain genetic information encoding the majority of the kappa 1b6 L chain gene locus. The protein sequence deduced from the kappa 1b6 C region gene was shown to differ from the published partial kappa 1b6 C region protein sequence at five amino acid positions. One of these differences results in a glycine to serine interchange that introduces an EcoRI restriction endonuclease recognition site within the kappa 1b6 C region gene. Subsequent genomic Southern blot analyses confirmed this structural assignment. Based on these data, the EcoRI-sensitive kappa-homologous fragments present within the genomes of the RMH H158 cell line and kappa 1b6 homozygous rabbits represent the nominal kappa 1 gene and not an alternative kappa isotype or kappa pseudogene. Rabbit Ig kappa 1 allelic nucleotide sequence homology comparisons have shown the isolated kappa 1b6 J-C gene locus to display common structural features previously identified in other kappa 1 alleles.

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Amino Acid Sequence Animals Base Sequence Cell Line Gene Expression Genes, Immunoglobulin Immunoglobulin Allotypes Immunoglobulin kappa-Chains Molecular Sequence Data Rabbits Restriction Mapping Sequence Homology, Nucleic Acid Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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K L Dreher, V Asundi, B Wolf, L Bruggeman. 1990-07-01. Rabbit Ig kappa 1b6 gene structure.. https://pubmed.ncbi.nlm.nih.gov/2113556/

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