Heavy-chain class switch does not terminate somatic mutation.

H Shan; M Shlomchik; M Weigert

doi:10.1084/jem.172.2.531

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Heavy-chain class switch does not terminate somatic mutation.

H Shan·M Shlomchik·M Weigert

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Abstract

We studied the H-chain class switch rearrangement in four groups of clonally related B cell hybridomas, to test the hypothesis that class switch terminates somatic mutation in a B cell. Using switch region-specific probes in Southern blot analysis individual mu-gamma switch rearrangement events can be distinguished. We show that clonally related IgG-producing hybridomas that differ by mutations often share a common switch rearrangement. This indicates that class switch in these cells did not terminate somatic mutation.

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Animals Antibodies, Monoclonal Autoimmune Diseases Blotting, Southern Cell Line DNA Gene Rearrangement Genes, Regulator Genes, Switch Immunoglobulin Heavy Chains Immunoglobulin Switch Region Liver Mice Mice, Mutant Strains Mutation Restriction Mapping Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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H Shan, M Shlomchik, M Weigert. 1990-08-01. Heavy-chain class switch does not terminate somatic mutation.. https://doi.org/10.1084/jem.172.2.531

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