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Excitotoxin lesion of nucleus basalis causes a specific decrease in Go mRNA in cerebral cortex. Sensitivity to MK-801.

H Wood·J de Belleroche
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Abstract

Lesions of the ascending cholinergic pathway from nucleus basalis are known to have profound effects on cortical function. In particular, a substantial potentiation of carbachol-stimulated polyphosphoinositide turnover is detected from 1 day after lesion and is maintained for several days before returning to normal by 1 month. In this study the effect of this lesion was investigated on levels of three G-protein alpha-subunit mRNAs. Excitotoxin lesion of the nucleus basalis caused a selective reduction in the levels of Go alpha mRNA in cerebral cortex ipsilateral to the lesion, Gs alpha and Gi alpha mRNA being unaffected. The maximal effect was obtained at 3 days after lesion where levels of Go alpha mRNA were decreased by 40% compared to sham-operated animals. Levels of Go alpha mRNA returned to normal values by 28 days. Treatment with MK-801 caused a significant attenuation of the decrease in Go alpha mRNA, indicating the involvement of NMDA receptors in this response.

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AnimalsAnticonvulsantsBlotting, NorthernBrainCerebral CortexDizocilpine MaleateFunctional LateralityGTP-Binding ProteinsKainic AcidKineticsMaleRNA, MessengerRatsRats, Inbred StrainsReference ValuesTime FactorsJournal ArticleResearch Support, Non-U.S. Gov't

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H Wood, J de Belleroche. 1990-10-29. Excitotoxin lesion of nucleus basalis causes a specific decrease in Go mRNA in cerebral cortex. Sensitivity to MK-801.. https://doi.org/10.1016/0014-5793(90)81051-o

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