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cDNA clone encoding Drosophila transcription factor TFIID.

M L Muhich·C T Iida·M Horikoshi·R G Roeder·C S Parker
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Abstract

Proper initiation of transcription by RNA polymerase II requires the TATA-consensus-binding transcription factor TFIID. A cDNA clone encoding the Drosophila TFIID protein has been isolated and characterized. The deduced amino acid sequence reveals an open reading frame of 353 residues. The carboxyl-terminal 180 amino acids are approximately 80% identical to yeast TFIID and 88% identical to human TFIID. The amino-terminal portions of the yeast and Drosophila TFIID proteins lack appreciable homology, whereas the Drosophila and human amino termini appear qualitatively similar. In addition, the amino-terminal region of the Drosophila TFIID contains several sequence motifs that are found in other Drosophila proteins which appear to regulate transcription.

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Amino Acid SequenceAnimalsBase SequenceCloning, MolecularDNADrosophila melanogasterEmbryo, NonmammalianHumansMolecular Sequence DataNucleic Acid HybridizationPolymerase Chain ReactionSaccharomyces cerevisiaeSequence Homology, Nucleic AcidTATA BoxTranscription Factor TFIIDTranscription FactorsTranscription, GeneticComparative StudyJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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M L Muhich, C T Iida, M Horikoshi, R G Roeder, C S Parker. 1990. cDNA clone encoding Drosophila transcription factor TFIID.. https://doi.org/10.1073/pnas.87.23.9148

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