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Enzyme immunoassay for flecainide.

J P Freche·G Coudert·M Wellman·G Siest
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Abstract

In order to develop an enzyme immunoassay (ELISA) for measurement of plasma flecainide levels, we first synthesized flecainide-hemisuccinate-BSA as immunogen. A highly specific antiserum was then raised in rabbits. Enzyme-labelled flecainide was prepared by the mixed anhydride method using hemifumarate-flecainide and horse radish peroxidase. The limit of detection was 10 ng/ml, the method was linear up to 1,000 micrograms/l. The intra and inter-assay variation was 5.1-7.8%. Cross reactivity with various metabolites of flecainide or some chemically related drugs was less than 0.1%. By contrast the cross reaction with some structural analogues achieved 25%. The importance of parts of the aromatic ring structure in immunogenic properties of this drug is discussed.

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AntibodiesAntibody SpecificityAntigensFlecainideHumansImmunoenzyme TechniquesMolecular StructureSerum Albumin, BovineSuccinic AnhydridesComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

J P Freche, G Coudert, M Wellman, G Siest. 1990-10-15. Enzyme immunoassay for flecainide.. https://doi.org/10.1016/0009-8981(90)90167-q

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