Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor.

J C Miller; Y Wang; D Filer

doi:10.1016/0006-291x(90)91917-h

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Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor.

J C Miller·Y Wang·D Filer

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Abstract

A rat brain cDNA library constructed in lambda ZAP II was screened with three oligonucleotide probes based on the reported coding region of the D2 receptor gene, RGB-2. A complete cDNA clone, D2(8)-1, showing positive signals with the three probes was subsequently identified by restriction analysis and dideoxy sequence analysis to be a variant of the RGB-2 gene. Comparison of the two genes revealed almost complete homology except that D2(8)-1 contains an 87 bp insert within the protein coding region and 265 additional nucleotides 5' upstream from the 5' end reported for RGB-2. It is suggested that at least two mRNA species encoding for D2 receptors exist in rat brain, possibly resulting from alternative splicing of RNA.

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Amino Acid Sequence Animals Base Sequence Brain Cloning, Molecular DNA Molecular Sequence Data Oligonucleotide Probes Rats Receptors, Dopamine Receptors, Dopamine D2 Restriction Mapping Journal Article Research Support, U.S. Gov't, P.H.S.

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J C Miller, Y Wang, D Filer. 1990-01-15. Identification by sequence analysis of a second rat brain cDNA encoding the dopamine (D2) receptor.. https://doi.org/10.1016/0006-291x(90)91917-h

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