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PubMed · 2138125

Phenotypic changes in a CD4+ lymphocyte subset correlate with a conversion from suppressor to helper inducer function.

A M Nesbitt·D B Jones·K Moore
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Abstract

The monoclonal antibodies WR16 (CD45RA) and WR19 were used to isolate two reciprocal subsets of CD4 lymphocytes by negative selection. The CD4+/WR16-/WR19+ subset exhibited helper-inducer activity in a pokeweed mitogen (PWM)-driven Ig secretion assay, whereas the CD4+/WR16+/WR19- subset showed suppressor activity. When the CD4+/WR16+/WR19- subset was cultured in phytohaemagglutinin (PHA) or purified protein derivative (PPD) there was increased expression of the antigen binding WR19 but the cells remained WR16 negative. Cultures of CD4+/WR16+/WR19- lymphocytes with PHA or PPD induced a phenotypic change that resulted in a population which was CD4+/WR16-/WR19+. Investigation of the functional activity of the CD4+/WR16+/WR19- subset, before and after activation and phenotypic change to WR16-/WR19+, indicated that the change in phenotype correlated with a functional conversion from suppressor to helper-inducer activity. These results suggest that the CD4+/WR16+/WR19- suppressor subset comprises cells that differentiate to WR16-/WR19+ helper-inducer cells after exposure to antigen or mitogen.

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CD4 AntigensCell DifferentiationCells, CulturedHumansImmunoglobulinsLymphocyte CooperationMitogensT-LymphocytesT-Lymphocytes, Helper-InducerT-Lymphocytes, RegulatoryJournal ArticleResearch Support, Non-U.S. Gov't

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A M Nesbitt, D B Jones, K Moore. 1990. Phenotypic changes in a CD4+ lymphocyte subset correlate with a conversion from suppressor to helper inducer function.. https://pubmed.ncbi.nlm.nih.gov/2138125/

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