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PubMed · 2138458

A continuous fluorimetric assay for ATPase activity.

U Banik·S Roy
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Abstract

A new continuous coupled fluorimetric assay is described for ATPases in general. Thus phosphate released from ATP hydrolysis is coupled to the nucleoside phosphorylase reaction using 7-methylguanosine as a fluorescent substrate for the nucleoside phosphorylase reaction. The hydrolysis of 7-methylguanosine leads to 7-methylguanine, which has lower quantum yield and hence can be used to monitor ATP hydrolysis continuously. The method has the potential to be extended to GTPase and nucleotidyltransferase assays.

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AnimalsHydrogen-Ion ConcentrationIn Vitro TechniquesMyosinsOsmolar ConcentrationPhosphatesRabbitsSpectrometry, FluorescenceJournal Article

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U Banik, S Roy. 1990-03-01. A continuous fluorimetric assay for ATPase activity.. https://pubmed.ncbi.nlm.nih.gov/2138458/

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