Organization and function of a dioxin-responsive enhancer.

J M Fisher; L Wu; M S Denison; J P Whitlock

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PubMed · 2161840

Organization and function of a dioxin-responsive enhancer.

J M Fisher·L Wu·M S Denison·J P Whitlock

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Abstract

The dioxin-responsive enhancer upstream of the CYP1A1 gene contains four copies of the recognition motif for the liganded Ah receptor. The results of deletion analyses, linker-scanning analyses, and the analysis of individual enhancer subdomains reveal that each copy of the motif contributes to the response of the enhancer to 2,3,7,8-tetrachlorodibenzo-p-dioxin. In the context of the dioxin-responsive enhancer, a GC box, representing the DNA binding site for Sp1 (or a related transcription factor), has no detectable intrinsic activity but enhances gene expression when linked to a recognition motif for the liganded Ah receptor, thereby producing a synergistic effect on enhancer function.

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Animals Base Sequence Chromosome Deletion Cytochrome P-450 Enzyme System Dioxins Enhancer Elements, Genetic Escherichia coli Gene Expression Genes Molecular Sequence Data Mutation Oligonucleotide Probes Plasmids Polychlorinated Dibenzodioxins Receptors, Aryl Hydrocarbon Receptors, Drug Restriction Mapping Journal Article Research Support, U.S. Gov't, P.H.S.

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J M Fisher, L Wu, M S Denison, J P Whitlock. 1990-06-15. Organization and function of a dioxin-responsive enhancer.. https://pubmed.ncbi.nlm.nih.gov/2161840/

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