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An improved method for isolating cardiac myocytes useful for patch-clamp studies.

K Yazawa·M Kaibara·M Ohara·M Kameyama
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Abstract

An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a G omega seal and subsequent whole-cell recording is easily performed using these cells.

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AnimalsCell SeparationCell SurvivalElectrophysiologyFemaleGuinea PigsHeartIn Vitro TechniquesMicrobial CollagenaseMyocardiumPeptide HydrolasesPotassiumJournal ArticleResearch Support, Non-U.S. Gov't

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K Yazawa, M Kaibara, M Ohara, M Kameyama. 1990. An improved method for isolating cardiac myocytes useful for patch-clamp studies.. https://doi.org/10.2170/jjphysiol.40.157

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