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A refined complement-enhanced neutralization test for detecting antibodies to Junin virus.

J G Barrera Oro·K T McKee·J Spisso·B G Mahlandt·J I Maiztegui
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Abstract

A refined, complement-enhanced, plaque-reduction neutralization test was developed for measuring neutralizing antibodies against Junin (Argentine hemorrhagic fever) virus. The assay measured neutralizing antibodies after natural as well as vaccine-induced Junin virus infections. Among vaccinated individuals, titers were 2-4-fold higher than those obtained with conventional assays, without loss of specificity. Enhanced sensitivity was achieved by using a standardized complement source (vs human or animal serum) for virus dilution, incubation of virus-serum mixtures at 36 degrees C for 2 h (vs overnight at 4 degrees C) prior to plaque assay, control of age and density of cell monolayers, and variation in overlay conditions.

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Analysis of VarianceAnimalsAntibodies, ViralArenaviruses, New WorldHemorrhagic Fever, AmericanHumansNeutralization TestsVero CellsViral VaccinesJournal Article

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BibTeXRIS

J G Barrera Oro, K T McKee, J Spisso, B G Mahlandt, J I Maiztegui. 1990. A refined complement-enhanced neutralization test for detecting antibodies to Junin virus.. https://doi.org/10.1016/0166-0934(90)90009-5

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