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Tn10-derived transposons active in Bacillus subtilis.

M A Petit·C Bruand·L Jannière·S D Ehrlich
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Abstract

Small derivatives of the Escherichia coli transposon Tn10, comprising IS10 ends and a chloramphenicol resistance gene, were introduced in Bacillus subtilis on a thermosensitive plasmid, pE194. In the presence of the Tn10 transposase gene fused to signals functional in B. subtilis, these derivatives transposed with a frequency of 10(-6) per element per generation. They had no highly preferred insertion site or region, as judged by restriction analysis of the chromosomal DNA, and generated auxotrophic and sporulation-deficient mutants with a frequency of about 1%. These results suggest that Tn10 derivatives might be a useful genetic tool in B. subtilis and possibly other gram-positive microorganisms.

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Bacillus subtilisBase SequenceBlotting, SouthernDNA Transposable ElementsDNA, BacterialMolecular Sequence DataPlasmidsRecombination, GeneticRestriction MappingJournal ArticleResearch Support, Non-U.S. Gov't

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BibTeXRIS

M A Petit, C Bruand, L Jannière, S D Ehrlich. 1990. Tn10-derived transposons active in Bacillus subtilis.. https://doi.org/10.1128/jb.172.12.6736-6740.1990

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