Novel fluorogenic substrates for phosphodiesterase I.

E Sato; M Yoshikawa; Y Kanaoka

doi:10.1248/cpb.38.2287

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PubMed · 2177681

Novel fluorogenic substrates for phosphodiesterase I.

E Sato·M Yoshikawa·Y Kanaoka

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Abstract

The fluorescence of 9,10-dioxa-syn-3,4,6,7-tetramethylbimane (bimane) was found to be quenched in the presence of guanosine 5'-monophosphate. By using this phenomenon, the bimane system was used for the fluorophor of substrates for phosphodiesterase I. Bimanes were coupled to 5'-guanylic acid and the resulting compounds were shown to be portent fluorogenic substrates for the assay of phosphodiesterase I.

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Bridged Bicyclo Compounds Bridged Bicyclo Compounds, Heterocyclic Fluorescent Dyes Guanosine Monophosphate Phosphodiesterase I Phosphoric Diester Hydrolases Substrate Specificity Journal Article

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E Sato, M Yoshikawa, Y Kanaoka. 1990. Novel fluorogenic substrates for phosphodiesterase I.. https://doi.org/10.1248/cpb.38.2287

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