A Mn2(+)-dependent ribozyme.

V Dange; R B Van Atta; S M Hecht

doi:10.1126/science.2185542

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PubMed · 2185542

A Mn2(+)-dependent ribozyme.

V Dange·R B Van Atta·S M Hecht

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Abstract

An RNA hairpin identical in sequence with the one formed during autocyclization of the 414-nucleotide Tetrahymena intervening sequence undergoes strand scission at a specific site in the presence of Mn2+. In addition to representing one of the smallest and simplest ribozymes possible, strand scission occurs readily under physiological conditions, is unaffected by the presence of Mg2+, and displays salt, pH, and temperature optima of potential use in exploiting Mn2+ as a regulatory switch in intact cells. The chemistry of strand scission of the RNA hairpin is described, as is the Mn2(+)-dependent solvolysis of a 231-nucleotide RNA transcript containing this structural motif.

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Animals Base Sequence Introns Manganese Molecular Sequence Data Nucleic Acid Conformation RNA Splicing RNA, Catalytic RNA, Ribosomal Tetrahymena Journal Article Research Support, Non-U.S. Gov't

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V Dange, R B Van Atta, S M Hecht. 1990-05-04. A Mn2(+)-dependent ribozyme.. https://doi.org/10.1126/science.2185542

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