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DNA recombination during PCR.

A Meyerhans·J P Vartanian·S Wain-Hobson
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Abstract

PCR co-amplification of two distinct HIV1 tat gene sequences lead to the formation of recombinant DNA molecules. The frequency of such recombinants, up to 5.4% of all amplified molecules, could be decreased 2.7 fold by a 6 fold increase in Taq DNA polymerase elongation time. Crossover sites mapped essentially to three discrete regions suggesting specific Taq DNA polymerase pause or termination sites. PCR mediated recombination may be a problem when studying heterogeneous genetic material such as RNA viruses, multigene families, or repetitive sequences. This phenomenon can be exploited to create chimeric molecules from related sequences.

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Amino Acid SequenceBase SequenceChimeraCloning, MolecularDNA, RecombinantGene AmplificationGene Products, tatGenes, ViralGenes, tatHIV-1Molecular Sequence DataOligonucleotide ProbesPlasmidsPolymerase Chain ReactionRecombination, GeneticSequence Homology, Nucleic Acidtat Gene Products, Human Immunodeficiency VirusComparative StudyJournal ArticleResearch Support, Non-U.S. Gov't

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A Meyerhans, J P Vartanian, S Wain-Hobson. 1990-04-11. DNA recombination during PCR.. https://doi.org/10.1093/nar%2F18.7.1687

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