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A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.

R Anand·J H Riley·R Butler·J C Smith·A F Markham
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Abstract

The construction of a yeast artificial chromosome (YAC) primary gridded library of 35,000 clones from human lymphoblastoid (48,XXXX) cell line DNA is described. The average YAC size is approximately 350kb representing a greater than 3.5 times coverage of the genome. The library is stored at -70 degrees C as gridded clones on nylon filters impregnated with 20% glycerol and as glycerol suspensions of individual clones in microtitre plates providing a prolonged multi-user potential. To date we have used 14 single copy probes to screen this library by colony hybridisation as well as PCR and have isolated between 1 and 5 YAC clones for every probe.

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Cell LineChromosome MappingChromosomes, FungalCloning, MolecularGene LibraryHumansRestriction MappingSaccharomyces cerevisiaeTransformation, GeneticJournal Article

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R Anand, J H Riley, R Butler, J C Smith, A F Markham. 1990-04-25. A 3.5 genome equivalent multi access YAC library: construction, characterisation, screening and storage.. https://doi.org/10.1093/nar%2F18.8.1951

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