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PubMed · 2189785

An improved method for yeast 2 microns plasmid curing.

W Xiao·G H Rank
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Abstract

SMR1-410, a dominant resistance marker, was cloned into the FLP gene of 2 microns DNA to produce the chimeric YEp vector pWX823B. Selection for SMR1-410 at high concentrations of sulfometuron methyl maintained pWX823 at high copy number and resulted in the rapid and efficient loss of native 2 microns DNA. Using this protocol approximately 15% of the cells monitored showed loss of 2 microns DNA. The curing methodology is more efficient and convenient than previous methods and has the added advantage of being applicable to wild-type prototrophic cells.

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Cloning, MolecularDrug Resistance, MicrobialGenetic MarkersPlasmidsSaccharomyces cerevisiaeSulfonylurea CompoundsJournal ArticleResearch Support, Non-U.S. Gov't

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W Xiao, G H Rank. 1990-04-16. An improved method for yeast 2 microns plasmid curing.. https://doi.org/10.1016/0378-1119(90)90037-r

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