Grass pollen allergens: detection on pollen grain surface using membrane print technique.

P M O'Neill; M B Singh; R B Knox

doi:10.1159/000235126

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Grass pollen allergens: detection on pollen grain surface using membrane print technique.

P M O'Neill·M B Singh·R B Knox

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Abstract

A microtechnique is described to visualize pollen allergens by making prints from the surface of mature grains on nitrocellulose membrane. The allergen Lol p I is detected by specific monoclonal antibodies or specific IgE from sera of grass-pollen-allergic patients, visualized by the immunoperoxidase method. This technique offers the potential to study the distribution of individual allergens in aerobiology samples by bright-field microscopy, and to determine the sensitivity spectra of allergic individuals to known and unknown pollen sources.

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Allergens Antibodies, Monoclonal Antigens, Plant Immunoenzyme Techniques Immunoglobulin E Immunologic Techniques Plant Proteins Poaceae Pollen Journal Article

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P M O'Neill, M B Singh, R B Knox. 1990. Grass pollen allergens: detection on pollen grain surface using membrane print technique.. https://doi.org/10.1159/000235126

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