Antibody exchange immunochemistry.

J A Hammarback; R B Vallee

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PubMed · 2198277

Antibody exchange immunochemistry.

J A Hammarback·R B Vallee

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Abstract

Antibodies are found to transfer rapidly between antigen samples attached to separate solid supports. The half-times for this antibody exchange at 20 and 37 degrees C were 7 and 1 h, respectively. Antibody exchange was exhibited by all 17 polyclonal and monoclonal antibodies tested and was readily detected by enzyme-linked immunosorbent assay, immunoblotting, or immunocytochemical assays. Taking advantage of this phenomenon, antibodies can be affinity-purified using less than a picomole of antigen and small amounts of antibody.

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Animals Antibodies, Monoclonal Antigen-Antibody Reactions Brain Chemistry Cattle Cell Line Cytosol Immunohistochemistry Immunologic Techniques Kinetics Microtubule-Associated Proteins Microtubules Tubulin Journal Article Research Support, U.S. Gov't, P.H.S.

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J A Hammarback, R B Vallee. 1990-08-05. Antibody exchange immunochemistry.. https://pubmed.ncbi.nlm.nih.gov/2198277/

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