Alpha-interferon structure and natural killer cell stimulatory activity.

B L Li; X X Zhao; X Y Liu; H S Kim; K Raska; J R Ortaldo; B Schwartz; S Pestka

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PubMed · 2201435

Alpha-interferon structure and natural killer cell stimulatory activity.

B L Li·X X Zhao·X Y Liu·H S Kim·K Raska·J R Ortaldo·B Schwartz·S Pestka

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Abstract

Expression vectors for human alpha-interferon (Hu-IFN-alpha) J1, a site-specific mutant [Ser116]Hu-IFN-alpha J1, and Hu-IFN-alpha J/C or Hu-IFN-alpha C/J hybrids were constructed and expressed in Escherichia coli. These interferons and others were purified by immunoaffinity chromatography with a monoclonal antibody against human alpha-interferon. Their antiviral activity and ability to stimulate natural killer cell activity were determined in comparison to several other human interferons. These results provide some insight into structure-activity relationships for stimulating natural killer cells and confirm our previous conclusions that antiviral activity cannot be used to predict other activities for an individual IFN-alpha species. The observations suggest that the tertiary structure rather than any specific linear sequence of amino acids regulates the ability of the interferons to stimulate natural killer cell activity.

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Amino Acid Sequence Base Sequence Cloning, Molecular Cytotoxicity, Immunologic Escherichia coli Humans Interferon Type I Killer Cells, Natural Molecular Sequence Data Mutation Oligonucleotide Probes Plasmids Recombinant Proteins Restriction Mapping Structure-Activity Relationship Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

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B L Li, X X Zhao, X Y Liu, H S Kim, K Raska, J R Ortaldo, B Schwartz, S Pestka. 1990-09-01. Alpha-interferon structure and natural killer cell stimulatory activity.. https://pubmed.ncbi.nlm.nih.gov/2201435/

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