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A knowledge-based experimental design system for nucleic acid engineering.

K Jiang·J Zheng·S B Higgins·D M Watterson·T A Craig·T J Lukas·L J Van Eldik
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Abstract

Presented in this paper is a knowledge-based experimental design system that incorporates the domain expertise used in nucleic acid engineering, thus automating the processing of error-prone, laborious low-level work, and many decision-making steps, and guiding the biologist toward a workable plan. This allows the biologist to work at a higher abstraction level, concentrating on more fundamental, difficult and challenging problems directly related to protein structure - function relationships. Cassette-based site-directed mutagenesis and synthetic gene designs are used as examples to illustrate the utility of the knowledge-based system approach to experimental design.

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AlgorithmsAmino Acid SequenceArtificial IntelligenceBase SequenceDNA Mutational AnalysisMicrocomputersMolecular Sequence DataProtein EngineeringRestriction MappingS100 ProteinsSoftwareUser-Computer InterfaceJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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K Jiang, J Zheng, S B Higgins, D M Watterson, T A Craig, T J Lukas, L J Van Eldik. 1990. A knowledge-based experimental design system for nucleic acid engineering.. https://doi.org/10.1093/bioinformatics%2F6.3.205

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