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NADP+ reduction by human lymphocytes.

A Klein·A W Chan·B U Caplan·A Malin
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Abstract

The hexose monophosphate shunt (HMPS) is known to be responsible for the reduction of NADP+ by lymphocytes. We tried to find other enzymatic systems that might provide the lymphocytes with NADPH. By measuring the absorbance at 340 nm we noted that the addition of NADP+ to a preparation of disrupted lymphocytes resulted in the formation of NADPH at a rate of 4 nmol/10(6) cells per min. This phenomenon could not be changed by negative feedback inhibition of HMPS, and could not be attributed to the low concentration of glucose, glucose-6-phosphate (G-6-P) and isocitrate found in the cell preparation (NADP(+)-dependent isocitrate dehydrogenase in addition to HMPS NADP+ reducing enzymes was found to be present in lymphocytes). Because of the activity of a NADP(+)-dependent lactate dehydrogenase, pyruvate oxidized the NADPH as it was being formed. Here we demonstrate the presence of an unknown NADP+ reducer in lymphocytes which seems to play an additional role to HMPS in NADP+ reduction by lymphocytes. NADP(+)-dependent lactate dehydrogenase may play a role in regulating the NADP+/NADPH ratio.

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HumansL-Lactate DehydrogenaseLymphocytesNADPOxidation-ReductionPentose Phosphate PathwayPyruvatesPyruvic AcidJournal Article

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BibTeXRIS

A Klein, A W Chan, B U Caplan, A Malin. 1990. NADP+ reduction by human lymphocytes.. https://doi.org/10.1111/j.1365-2249.1990.tb05422.x

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