PubMed Health⌕ Search
Home/Archive/PubMed

PubMed · 2213340

Evaluation of a microcomputed tomography system to study trabecular bone structure.

J L Kuhn·S A Goldstein·L A Feldkamp·R W Goulet·G Jesion
View original source ↗Export metadata ↓

Abstract

A new microcomputed tomography (micro-CT) system and thresholding procedure was evaluated as a tool for nondestructive analysis of trabecular bone. Images of 6-mm trabecular bone cubes acquired from the micro-CT system were compared with optical images of corresponding histologic sections to determine the accuracy of representation. The stereologic measures of bone volume fraction (PP) and trabecular plate density (PL) were used to quantify the comparisons. The results showed that the micro-CT measures of PP were not significantly different from those measured from histologic sections and therefore were very accurate. Measures of PL were different by approximately 14%, which translated into discrepancies in trabecular plate thicknesses of about 19 microns. This difference was significantly correlated to the microstructural characteristics of the specific specimen scanned. The precision of both measurements was excellent.

Explore related subjects

Bone and BonesDifferential ThresholdMicrocomputersTomography, X-Ray ComputedJournal ArticleResearch Support, U.S. Gov't, P.H.S.

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

J L Kuhn, S A Goldstein, L A Feldkamp, R W Goulet, G Jesion. 1990. Evaluation of a microcomputed tomography system to study trabecular bone structure.. https://doi.org/10.1002/jor.1100080608

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related citations

PubMed2007

Cytogenetic and molecular cytogenetic findings in dedifferentiated liposarcoma with neural-like whorling pattern and metaplastic bone formation.

Dedifferentiated liposarcoma is a subtype of liposarcoma characterized by juxtaposition of well-differentiated liposarcoma with a nonadipocytic sarcoma. A peculiar form of dedifferentiated liposarcoma has been described, characterized by a nonlipogenic component with a neural-like whorling pattern of growth and metaplastic bone formation. We report the cytogenetic and molecular genetic findings of this peculiar form of dedifferentiation in a retroperitoneal tumor found in a 58-year-old female. The neoplasm had typical histologic findings and a complex karyotype characterized by several numeric and structural chromosomal abnormalities, including the presence of ring and giant rod chromosomes. Molecular cytogenetic studies found high levels of amplification of the MDM2 oncogene, consistent with the amplification of the 12q14 chromosome band, a cytogenetic abnormality commonly found in these tumors. These findings indicate that, despite its unique and peculiar morphologic features, this unusual type of dedifferentiated liposarcoma shares many of the cytogenetic features and molecular genetic abnormalities found in other forms of dedifferentiation. The specific cytogenetics and molecular determinants of these peculiar morphologic findings, however, remain unknown.

Bone and Bones↗
PubMed2007

Traditional and virtual microscopy compared experimentally in a classroom setting.

The technology known as virtual microscopy is now widely available to medical students. A number of medical school histology and pathology course directors, including those at the Eastern Virginia Medical School, are exploring the question of how best to make use of this new tool. The current study compared the efficacy of teaching and testing one unit of histology, bone and cartilage, using two technologies, namely, virtual microscopy and traditional microscopy. Additionally, the study examined whether low, moderate, high moderate, or high achieving students perform more effectively in any of the learning/testing formats. In a completely randomized block design, 96 first semester, first-year medical students were grouped by previous exam quartiles then subsequently randomly assigned to one of four groups. Using identical laboratory exercises with highly comparable slides for each experimental group, half of the subjects learned the exercise with traditional microscopy and half learned with virtual microscopy. Subjects were further randomly subdivided into virtual or actual testing groups. The authors found no significant differences in test scores when they examined effects by learning group or by testing group, nor were there significant interaction effects. Student performance evaluated by previous exam quartile was significant (P < 0.001). That is, students who had scored in a particular quartile on a previous test tended to score in the same quartile on the bone and cartilage test regardless of learning or testing method. In a short opinion survey, students were polled to evaluate their experience. Student preferences for both learning and testing method varied widely.

Bone and Bones↗