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PubMed · 221908

Solubilized and insolubilized bone morphogenetic protein.

M R Urist·A Mikulski·A Lietze
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Abstract

A bone morphogenetic protein (BMP) obtained in solution by digestion of demineralized rabbit cortical bone matrix with bacterial collagenase retains its biologically active conformation in a neutral salt/ethylene glycol mixture. BMP may be insolubilized by coprecipitation with calcium phosphate and resolubilized by chemical extraction with a neutral salt in the same solvent mixture. Upon concanavalin A-Sepharose chromatography, BMP is bound by hydrophobic interaction and carbohydrate recognition and is recovered by elution with either alpha-methyl mannoside or ethylene glycol solvent mixture. Implants of both eluates and the extracts of the coprecipitate in double-walled diffusion chambers induce transmembrane bone morphogenesis. BMP is not species specific; rabbit BMP induces new bone formation in the rat. The present observations indicate that BMP is a glycoprotein.

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AnimalsBiological AssayBone and BonesMicrobial CollagenaseProtein ConformationProteinsRabbitsSolubilityJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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M R Urist, A Mikulski, A Lietze. 1979. Solubilized and insolubilized bone morphogenetic protein.. https://doi.org/10.1073/pnas.76.4.1828

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