A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence.

J Jenkins; R G Hiller; J Speirs; J Godovac-Zimmermann

doi:10.1016/0014-5793(90)81082-y

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A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence.

J Jenkins·R G Hiller·J Speirs·J Godovac-Zimmermann

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Abstract

A genomic library of Chroomonas (Cryptophyceae) DNA has been constructed in lambda EMBL4. Using a synthetic oligomer as a hybridization probe, a clone containing a phycoerythrin alpha-subunit has been obtained and sequenced. The principal alpha 1- and alpha 2-subunits of the holoprotein have been partially sequenced by sequential Edman degradation and differ from the DNA derived sequence, providing evidence for at least 3 alpha-subunit genes. The nucleotide sequence of the alpha-subunit gene is GC rich and encodes an N-terminal extension which is putatively thylakoid-lumen directing.

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Amino Acid Sequence Base Sequence Cloning, Molecular Eukaryota Genes, Plant Macromolecular Substances Molecular Sequence Data Phycoerythrin Plasmids Restriction Mapping Sequence Homology, Nucleic Acid Comparative Study Journal Article Research Support, Non-U.S. Gov't

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J Jenkins, R G Hiller, J Speirs, J Godovac-Zimmermann. 1990-10-29. A genomic clone encoding a cryptophyte phycoerythrin alpha-subunit. Evidence for three alpha-subunits and an N-terminal membrane transit sequence.. https://doi.org/10.1016/0014-5793(90)81082-y

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