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Electrophoretic karyotyping without the need for generating protoplasts.

K McCluskey·B W Russell·D Mills
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Abstract

Chromosome samples for pulsed-field electrophoresis have been prepared without first generating protoplasts. The technique involves treatment of intact, agarose-solidified cell material with protease in the presence of EDTA and SDS. Saccharomyces cerevisiae, Ustilago hordei, Tilletia caries, and T. controversa karyotypes are clearly resolved with this technique. Colonies of U. hordei and S. cerevisiae removed from the surface of agar-solidified media and prepared for PFGE by this abbreviated method also yield well resolved karyotypes.

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Chromosomes, FungalElectrophoresis, Agar GelFungiKaryotypingProtoplastsJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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K McCluskey, B W Russell, D Mills. 1990. Electrophoretic karyotyping without the need for generating protoplasts.. https://doi.org/10.1007/bf00318221

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