[Study of sarcoplasmic reticulum Ca-ATPase by the paramagnetic label--paramagnetic probe method].

R A Kotel'nikova; L V Tat'ianenko; A V Kulikov; A I Kotel'nikov; A V Mel'nikov

PubMed · 226172

[Study of sarcoplasmic reticulum Ca-ATPase by the paramagnetic label--paramagnetic probe method].

R A Kotel'nikova·L V Tat'ianenko·A V Kulikov·A I Kotel'nikov·A V Mel'nikov

Abstract

ESR spectra of nitroxyl derivative labelling with parachlormercury benzoate bound with SH-groups of Ca, Mg-dependent ATPase in the presence of Mn2+ ions are studied. It has been concluded from the saturation curves of ESR spectra that Mn2+ is localized at the distance approximately 40 A from the SH-group of the enzyme active centre. Platinum compound (K2PtCl4) changes the spectrum of ESR 1 due to the displacement of the label from the enzyme SH-groups and disintegration of the sarcoplasmic reticulum structure. Palladium compound (Pd(NH3)4Cl2) produced no effect on the ESR 1 spectrum.

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Animals Binding Sites Calcium-Transporting ATPases Chemical Phenomena Chemistry Chloromercuribenzoates Electron Spin Resonance Spectroscopy Magnesium Manganese Palladium Platinum Rabbits Sarcoplasmic Reticulum Journal Article

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R A Kotel'nikova, L V Tat'ianenko, A V Kulikov, A I Kotel'nikov, A V Mel'nikov. [Study of sarcoplasmic reticulum Ca-ATPase by the paramagnetic label--paramagnetic probe method].. https://pubmed.ncbi.nlm.nih.gov/226172/

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