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Transcriptase activity associated with rabies virion.

A Kawai
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Abstract

Rabies virion-associated transcriptase activity was investigated in vitro and compared with that of the New Jersey serotype of vesicular stomatitis virus. The concentration of detergent that affected [3H]GMP incoporation into acid-insoluble material was significantly different for both viruses. Vesicular stomatitis virus New Jersey required 0.05 to 0.1% nonionic detergent, whereas rabies virion could not be fully activated unless 4 to 5% detergent was used. Other optimal conditions were as follows: 40 mM NaCl, 5 mM Mg2+, 40 mM Tris-hydrochloride (pH 7.4), 5 mM dithiothreitol, and 30 degrees C. The reaction required four nucleoside triphosphates. The initial rate of RNA synthesis by rabies virion enzyme was 140 pmol of GMP incorporated/mg of viral protein per h and linearly increased until about 8 h, with a slight initial lag phase. The enzyme activity that correlated with the content of L protein was highest when rabies virions were grown at 33 degrees C. The product was single-stranded RNA, which was complementary in base sequences to rabies viral RNA. Most of the RNA synthesized sedimented at 6-16S.

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DNA-Directed RNA PolymerasesDithiothreitolGuanosine MonophosphateHydrogen-Ion ConcentrationKineticsMagnesiumRNA, ViralRabies virusVesiculovirusVirionComparative StudyJournal Article

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BibTeXRIS

A Kawai. 1977. Transcriptase activity associated with rabies virion.. https://doi.org/10.1128/jvi.24.3.826-835.1977

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