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PubMed · 2280774

Calcitonin exon sequences influence alternative RNA processing.

G J Cote·I N Nguyen·S M Berget·R F Gagel
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Abstract

The pre-mRNA encoding calcitonin (CT) and CT gene-related peptide (CGRP) is differentially processed in a tissue-specific fashion to include exon 4 (which encodes CT) or exclude this exon and splice to exon 5 (which encodes CGRP). We have used a CT-specific in vitro RNA-processing system to identify cis-acting sequences required to prevent splicing to exon 5. Deletion mapping demonstrated the presence of an element within the first 45 nucleotides of the CT-specific exon 4 that was required to suppress splicing to the CGRP-specific exon 5. This element was able to function in a completely heterologous system to suppress splicing when the CGRP exon was replaced with a constitutive viral exon. The element was unable to suppress splicing in the absence of a proximal CT-specific 3' splice site. Our results suggest that CT-specific splicing requires assisted recognition of its 3' splice site.

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CalcitoninCalcitonin Gene-Related PeptideChromosome DeletionExonsGene Expression RegulationHeLa CellsHumansRNA Processing, Post-TranscriptionalRNA SplicingRNA, MessengerRegulatory Sequences, Nucleic AcidJournal ArticleResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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BibTeXRIS

G J Cote, I N Nguyen, S M Berget, R F Gagel. 1990. Calcitonin exon sequences influence alternative RNA processing.. https://doi.org/10.1210/mend-4-11-1744

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PubMed2006

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