Self-assembling bilayer lipid membranes on solid support.

H T Tien; Z Salamon

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PubMed · 2288700

Self-assembling bilayer lipid membranes on solid support.

H T Tien·Z Salamon

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Abstract

Solid-supported bilayer lipid membranes (s-BLMs) that possess some properties similar to those of conventional BLMs can be self-assembled on a freshly cleaved metal wire by a two-step procedure: (i) The tip of a Teflon-coated platinum wire, while immersed in a lipid solution, is cut off with a scalpel; (ii) the new tip of the wire, having become coated with lipid solution, is transferred into 0.1 M KCl. After a few minutes, a stable lipid bilayer forms spontaneously on the tip of the wire, as verified by electrical measurements. An application of such a supported BLM (s-BLM) is reported for the detection of Pb2+ ions. The s-BLM is liquid-crystalline in structure, which makes it amenable to modification for basic studies, as well as for technological applications such as biosensors and molecular electronic devices.

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Lipid Bilayers Methods Platinum Silver Stainless Steel Journal Article Research Support, U.S. Gov't, P.H.S.Review

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H T Tien, Z Salamon. 1990. Self-assembling bilayer lipid membranes on solid support.. https://pubmed.ncbi.nlm.nih.gov/2288700/

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