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Cloning of two hypothalamic cDNAs encoding tissue-specific transcripts in the preoptic area and testis.

F V Nowak
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Abstract

Neuropeptides are central to the regulation of normal sexual development and reproduction. Two new hypothalamic cDNAs have been identified by Northern blot analysis and molecular cloning. Each potentially encodes a precursor for a unique GnRH-like decapeptide. Northern blot analysis reveals tissue-specific transcripts from each gene in the hypothalamus and testis. These preoptic area regulatory factor genes, PORF-1 and PORF-2, may thus encode members of a family of GnRH-related peptides which have been described in these tissues.

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Amino Acid SequenceAnimalsBase SequenceCloning, MolecularDNAGonadotropin-Releasing HormoneHypothalamusMaleMolecular Sequence DataNucleic Acid HybridizationPreoptic AreaRNA, MessengerRatsRats, Inbred StrainsRegulatory Sequences, Nucleic AcidTestisTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.

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F V Nowak. 1990. Cloning of two hypothalamic cDNAs encoding tissue-specific transcripts in the preoptic area and testis.. https://doi.org/10.1210/mend-4-8-1205

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