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Polyamines and cellular adenosine 3' :5'-cyclic monophosphate.

C Clô·C M Caldarera·B Tantini·D Benalal·U Bachrach
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Abstract

The effect of polyamines on the cellular concentrations of cyclic AMP was studied. It was shown that 1 microM-spermine caused a decrease in cyclic AMP in chick-embryo heart cells, chick-embryo fibroblasts, neuroblastoma, glioma and neuroblastoma-glioma hybrid cells, grown in culture. A similar decrease was observed when polyamines were added to cells in the presence of a phosphodiesterase inhibitor or after stimulating the cells with various hormones. Noradrenaline was used in cultures of heart cells, prostaglandin E1 and adenosine for neuroblastoma and neuroblastoma-glioma hybrids, whereas isoproterenol was used for the stimulation of glioma cells. Polyamines at higher concentrations were either without effect or caused a slight increase in cyclic AMP. Spermidine (10 microM) also caused a decrease in cellular cyclic AMP, as did 0.1 microM-putrescine. It is suggested that the effect of polyamines on cellular cyclic AMP may be explained by the effect of these polycations on the activity of cellular phosphodiesterase.

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AdenosineAnimalsCells, CulturedChick EmbryoCyclic AMPFibroblastsGliomaHybrid CellsMyocardiumNeuroblastomaNorepinephrinePolyaminesRatsJournal Article

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C Clô, C M Caldarera, B Tantini, D Benalal, U Bachrach. 1979-09-15. Polyamines and cellular adenosine 3' :5'-cyclic monophosphate.. https://doi.org/10.1042/bj1820641a

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