Phosphorylated dolichols in aging.

R K Pullarkat; P S Pullarkat; G N Morris

doi:10.1042/bj2650891

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Phosphorylated dolichols in aging.

R K Pullarkat·P S Pullarkat·G N Morris

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Abstract

The age-associated changes in the levels and synthesis of dolichyl phosphate and dolichyl diphosphate derivatives were investigated in brain and liver of 057B1/NNia mice. The total chloroform/methanol (2:1, v/v)-extractable phosphorylated dolichols of brain increased from 1.01 micrograms/g at 3 months to 5.22 micrograms/g at 28 months of age. The long-chain dolichyl diphosphate oligosaccharide (Dol-PP-oligo) levels of brain increased from 0.82 microgram/g in 3 months to 2.8 micrograms/g in 28-month-old animals. However, in liver and in kidney, the levels of these components were unaffected by age. Incorporation of labelled glucose from UDP-glucose into dolichyl phosphate glucose and Dol-PP-oligo in brain microsomes was unaffected by age, whereas, in liver microsomes, the rates of synthesis of both components increased by 50-150%. The increased rate of synthesis and lack of accumulation of Dol-PP-oligo in liver suggest an active utilization and/or catabolism of these glycoprotein precursors. The accumulation of Dol-PP-oligo in aging brain may reflect its decreased utilization for N-glycosylation and/or reduced catabolism.

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Aging Animals Brain Chromatography, High Pressure Liquid Chromatography, Thin Layer Dolichol Phosphates Dolichols Glycoproteins Mice Mice, Inbred C57BL Microsomes Microsomes, Liver Phosphorylation Spectrophotometry, Ultraviolet Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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R K Pullarkat, P S Pullarkat, G N Morris. 1990-02-01. Phosphorylated dolichols in aging.. https://doi.org/10.1042/bj2650891

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